ELISA as a serological test for theileria annulata in Behera province Egypt

Enzyme linked immunosorbent assay [ELISA] was applied for the first time in Egypt using a local prepared schizont antigen for the detection of Theileria annulata among local breed cattle. The routine parasitological examination of Giemsa stained blood films was also used. The study revealed differences in the infection rates detected by ELISA [80.4%] and blood films examination [65.4%]. ELISA proved to be more accurate, practical, serodiagnostic and a suitable screening test for field detection of Theileria annulata infection, as it was possible to determine antibody levels in more serum samples daily

Evaluation of double diffusion test in the diagnosis of hydatidosis in livestock

The double diffusion test was used for detecting cross serological reactions between hydatid fluid antigens and sera of ruminants infested with different helminth parasites. The whole hydatid fluid antigen showed positive reactions with sera of sheep infested with Cysticercus tenuicollis, sera of cattle infested with Cysticercus bovis, sera of buffalos infested with Fasciola gigantica and Paramphistomum species and sera of cattle harbored Fasciola gigantica and Moniezia species. Sera of cattle infested with Paramphistomum species were negative. No cross reactions were detected when the purified antigen was used against the previously mentioned sera. The sensitivity of double diffusion test for diagnosis of hydatidosis using purified antigen was 96.6%

Trials with avian anticoccidials for treatment of coccidiosis in lambs

Twenty Baladi lambs aged 3-4 months were found naturally infected with coccidia. Six species of Eimeria were identified, which are E. ninakohlyakimovae, E. parva, E. intricata, E. granulosa, E. cr and allis, and E. ovina. Trials for treatment of such infestation were made using one old traditional anticoccidial drug [Amprolium] and two recent preparations, which are AS supermed [combination of Amprolium and sulphaquinoxaline], Baycox [Toltrazuril]. The criteria used for the efficacy of such drugs are oocyst discharge and body gain. It was found after the statistical analysis that, Amprolium is still the drug of choice in treatment of coccidiosis in lambs. On the other h and, the efficacy of Baycox and AS supermed is quite equal in decreasing oocyst discharge and increasing body weight of treated lambs